FluA/ FluB/ SARS-CoV-2 Real-time RT-PCR Kit

Getein FluA/ FluB/ SARS-CoV-2 Real-time RT-PCR Kit is used to quickly identify whether a patient is infected with influenza A, influenza B or SARS-CoV-2, thereby effectively controlling viral infection.
  • Test Item:

    FluA/FluB/SARS-CoV-2 Real-time RT-PCR Kit
  • Sample Type:

    Serum/Plasma/Whole Blood/Fingertip blood
  • Test Time:

    15 min
  • Methodology:

    Colloidal Gold

FluA/ FluB/ SARS-CoV-2 Real-time RT-PCR Kit




Intended Use

FluA/ FluB/ SARS-CoV-2 Real-time RT-PCR Kit is a multiple real-time RT-PCR test intended for the qualitative detection of nucleic acid from influenza A, influenza B or SARS-CoV-2 virus in oropharyngeal swab and nasopharyngeal swab samples of suspected cases. This test is only for laboratory use, not for home testing.


About FluA/ FluB/ SARS-CoV-2 Real-time RT-PCR

Influenza is a highly contagious, acute respiratory illness characterized by the abrupt onset of fever, myalgia, headache, malaise, nonproductive cough, sore throat, and rhinitis. Influenza viruses are negative-strand RNA viruses, and the viruses consist of three types: Influenza A, Influenza B and Influenza C. Human influenza A and B viruses cause seasonal epidemics of disease (known as the flu season) almost every winter, and their clinical symptoms are similar.

The novel coronaviruses belong to the β genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source of infection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.


Contents

No.

Contents

Volume

Qty

Main ingredients

24 Tests/kit

48 Tests/kit

1

Flu A/Flu B/SARS-CoV-2-PCR

Master Mix (Lyophilized Powder)

520 μL/bottle

1000 μL/bottle

1


Specific primers and probes, RT-PCR Buffer,
Rnasin, Hot-start Taq enzyme, RT enzyme,
Protective agent for lyophilization, etc.


2


Flu A/Flu B/SARS-CoV-2 Positive
Control (Lyophilized Powder)


500 μL/bottle

500 μL/bottle

1


In vitro transcription RNA containing target
genes (M1/M2, NEP/NS1, ORF lab and N
genes) and internal standard gene fragments


3

Flu A/Flu B/SARS-CoV-2 Diluent

1500 μL/bottle

2000 μL/bottle

1

DEPC Water

Note: The above components of different batch kits cannot be used interchangeably.


Specifications


Test Item

Sample Type

FluA/FluB/SARS-CoV-2 Real-time RT-PCR

Oropharyngeal swab/

Nasopharyngeal swab

Storage Condition

Shelf Life

-20°C

6 months


Storage and Stability


Steps

Temperature

Time

Cycles

Reverse Transcription

48°C

10 min

1

cDNA pre-denaturation

95°C

2 min

1

Denaturation

95°C

10 s

45

Annealing, extension, fluorescence acquisition

55°C

30 s


Clinical Application

1. The kit can quickly identify whether a patient is infected with influenza A, influenza B or SARS-CoV-2, thereby effectively controlling viral infection.

2. The test results of this kit are for clinical reference only and should not be used as the only standard for clinical diagnosis. It is recommended to combine the patient's clinical manifestations and other laboratory tests for a comprehensive analysis of the condition.


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